DNA

Part:BBa_K5109002:Design

Designed by: Lisa Faccincani   Group: iGEM24_Uni-Padua-IT   (2024-09-24)


DNA sequence for Golden Gate assembly.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 27


Design Notes

During the course of our project, we only used RFC 10 assembly, and not Golden Gate. The part does not interfere with RFC 10 assembly.



Source

The part was designed on benchling and synthesized in the contest of a full expression cassette sequence.

References